6ANO
Crystal structure of human FLASH N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9774 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.800, 43.750, 65.000 |
| Unit cell angles | 90.00, 114.51, 90.00 |
Refinement procedure
| Resolution | 35.508 - 2.610 |
| R-factor | 0.2021 |
| Rwork | 0.199 |
| R-free | 0.25150 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.651 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXCD |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.508 | 2.770 |
| High resolution limit [Å] | 2.600 | 2.610 |
| Rmerge | 0.055 | 0.493 |
| Number of reflections | 10151 | |
| <I/σ(I)> | 14.24 | 2.72 |
| Completeness [%] | 99.3 | 98.3 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.846 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100 mM Tris (pH 8.0), 18% (w/v) PEG 4000 |
