6ANH
Crystal structure of PPK2 class III in complex with Guanosine 5-tetraphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 110.812, 110.812, 178.807 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.170 - 2.650 |
R-factor | 0.19616 |
Rwork | 0.194 |
R-free | 0.23813 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.493 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.200 | 2.740 |
High resolution limit [Å] | 2.600 | 2.650 |
Rmerge | 0.080 | |
Number of reflections | 17542 | |
<I/σ(I)> | 25 | |
Completeness [%] | 99.8 | |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.1 M Ammonium tartrate dibasic |