6AND

Pinatuzumab Fab in complex with anti-Kappa VHH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-10
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	63.402, 72.947, 70.724
Unit cell angles	90.00, 110.73, 90.00

Refinement procedure

Resolution	46.012 - 1.750
R-factor	0.2425
Rwork	0.241
R-free	0.28990
RMSD bond length	0.003
RMSD bond angle	0.690
Data reduction software	XPREP
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.142	1.920	1.850
High resolution limit [Å]	1.750	1.870	1.750
Number of reflections	57457
<I/σ(I)>	16.05
Completeness [%]	93.6
Redundancy	3.35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M lithium sulfate, 0.1 M HEPES pH 7.4 and 25 % polyethyleneglycol (PEG) 3350