6ANA
LL2 Fab in complex with anti-Kappa VHH domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-18 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 |
Unit cell lengths | 38.925, 63.682, 68.417 |
Unit cell angles | 114.28, 96.14, 91.92 |
Refinement procedure
Resolution | 38.559 - 1.700 |
R-factor | 0.2154 |
Rwork | 0.215 |
R-free | 0.23650 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.824 |
Data reduction software | XPREP |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.559 | 1.720 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.051 | 0.362 |
Rpim | 0.036 | 0.258 |
Number of reflections | 58574 | |
<I/σ(I)> | 15.8 | |
Completeness [%] | 89.6 | |
Redundancy | 2.7 | |
CC(1/2) | 0.998 | 0.765 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 1M lithium chloride 0.1M MES, pH 6.0 |