6AMT
Human Class I MHC HLA-A2 in complex with synthetic peptide MMWDRGLGMM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.297, 84.930, 84.322 |
Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
Resolution | 19.950 - 2.496 |
R-factor | 0.2156 |
Rwork | 0.211 |
R-free | 0.24940 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.453 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 5.350 | 2.500 |
Rmerge | 0.135 | 0.133 | 0.550 |
Rmeas | 0.165 | 0.161 | 0.690 |
Rpim | 0.094 | 0.089 | 0.411 |
Total number of observations | 84652 | ||
Number of reflections | 28129 | 2878 | 2721 |
<I/σ(I)> | 13.5 | ||
Completeness [%] | 98.5 | 98.8 | 97.6 |
Redundancy | 3 | 2.9 | 3 |
CC(1/2) | 0.955 | 0.630 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | 25% PEG1000, 0.01% sodium azide, 0.1 M MES |