6AMM
CAT192 Fab Insertion Mutant H0/L1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-14 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5419 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 72.790, 65.720, 110.448 |
| Unit cell angles | 90.00, 102.83, 90.00 |
Refinement procedure
| Resolution | 35.350 - 2.800 |
| R-factor | 0.2522 |
| Rwork | 0.247 |
| R-free | 0.30250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6amj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.809 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.350 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.497 | |
| Number of reflections | 12663 | 1837 |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 100 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 20% PEG 8K, 0.1M Sodium cacodylate pH 6.5, 0.2M MgCl2 |
