6AMG

cyt P460 of Nitrosomonas sp. AL212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979100
Spacegroup name	P 21 21 21
Unit cell lengths	47.267, 80.138, 108.962
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.558 - 1.450
R-factor	0.1417
Rwork	0.141
R-free	0.15750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2je2
RMSD bond length	0.028
RMSD bond angle	2.348
Data reduction software	XDS
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	65.558
High resolution limit [Å]	1.402
Rmerge	0.041
Number of reflections	77014
<I/σ(I)>	18.4
Completeness [%]	94.0
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M NaCl, 0.1 M sodium acetate pH 5.0, PEG 6000