6AMF
Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/L36E at cryogenic temperature
Replaces: 3QONExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2017-07-27 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.140, 60.316, 38.567 |
| Unit cell angles | 90.00, 94.32, 90.00 |
Refinement procedure
| Resolution | 38.460 - 1.850 |
| R-factor | 0.2111 |
| Rwork | 0.209 |
| R-free | 0.26050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.832 |
| Data reduction software | SAINT |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.460 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.028 | 0.167 |
| Number of reflections | 12204 | 1224 |
| <I/σ(I)> | 35.27 | 4.66 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 24% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
