6AME
TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 M21A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.592, 39.525, 45.932 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| R-factor | 0.166 * |
| Rwork | 0.171 |
| R-free | 0.23700 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1msi |
| RMSD bond length | 0.016 |
| RMSD bond angle | 27.660 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.075 * | |
| Total number of observations | 11961 * | |
| Number of reflections | 3554 | |
| <I/σ(I)> | 15.9 | 7.2 |
| Completeness [%] | 94.7 | 91.5 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.5 | 20 * | Jia, Z., (1995) Protein Sci., 4, 1236. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3-3.5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 25-27.5 (%sat) | |
| 3 | 1 | drop | sodium acetate | 0.05 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 50-55 (%sat) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) |
