6AM3
Regulator of G protein signaling (RGS) 17 in complex with Ca2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 115 |
| Detector technology | PIXEL |
| Collection date | 2017-03-08 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.078 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.571, 59.295, 65.337 |
| Unit cell angles | 90.00, 100.60, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.530 |
| R-factor | 0.2043 |
| Rwork | 0.203 |
| R-free | 0.22470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zv4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.310 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.560 |
| High resolution limit [Å] | 1.530 | 4.150 | 1.530 |
| Rmerge | 0.132 | 0.056 | 0.665 |
| Rmeas | 0.146 | 0.060 | 0.846 |
| Rpim | 0.061 | 0.022 | 0.517 |
| Total number of observations | 190012 | ||
| Number of reflections | 39255 | 2321 | 921 |
| <I/σ(I)> | 4.6 | ||
| Completeness [%] | 87.5 | 99.9 | 41.4 |
| Redundancy | 4.8 | 7.2 | 1.7 |
| CC(1/2) | 0.997 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.2 M calcium chloride dihydrate, 0.1 M MES, pH 6.0, 22% PEG3350 |
