6ALX
Structure of F. tularensis MglA-SspA solved in the presence of polyP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-08-08 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.253, 114.298, 141.651 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.753 - 3.300 |
R-factor | 0.2177 |
Rwork | 0.209 |
R-free | 0.29440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5u56 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.754 | 3.480 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmeas | 0.117 | 0.643 |
Rpim | 0.062 | 0.280 |
Number of reflections | 16079 | |
<I/σ(I)> | 8.1 | 2.5 |
Completeness [%] | 94.2 | 100 |
Redundancy | 3.5 | 3.3 |
CC(1/2) | 0.994 | 0.843 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | Peg 3350 |