6ALP

VioC L-arginine hydroxylase bound to Fe(II), 3S-hydroxy-L-arginine, and succinate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	80.770, 66.836, 62.925
Unit cell angles	90.00, 109.21, 90.00

Refinement procedure

Resolution	59.420 - 1.990
R-factor	0.2189
Rwork	0.217
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.850
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.420	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.136	0.037	0.757
Rmeas	0.158	0.044	0.888
Rpim	0.081	0.023	0.462
Total number of observations	80631
Number of reflections	21375	1118	1092
<I/σ(I)>	5.9
Completeness [%]	98.5	99.6	97
Redundancy	3.8	3.7	3.5
CC(1/2)		0.997	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1 M succinic acid, 0.1 M Tris pH 8.0