6ALO
VioC L-arginine hydroxylase bound to Fe(II), L-arginine, and a peroxysuccinate intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.760, 66.653, 62.889 |
| Unit cell angles | 90.00, 109.21, 90.00 |
Refinement procedure
| Resolution | 59.390 - 1.790 |
| R-factor | 0.1766 |
| Rwork | 0.175 |
| R-free | 0.20340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.063 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 59.390 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.790 | 4.880 | 1.800 |
| Rmerge | 0.050 | 0.020 | 0.492 |
| Rmeas | 0.058 | 0.023 | 0.579 |
| Rpim | 0.030 | 0.012 | 0.302 |
| Number of reflections | 29761 | 1537 | 1462 |
| <I/σ(I)> | 10.8 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 3.8 | 3.7 | 3.6 |
| CC(1/2) | 0.999 | 0.865 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1 M 2-oxoglutarate, 0.1 M Tris pH 8.5 |
