6ALO

VioC L-arginine hydroxylase bound to Fe(II), L-arginine, and a peroxysuccinate intermediate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	80.760, 66.653, 62.889
Unit cell angles	90.00, 109.21, 90.00

Refinement procedure

Resolution	59.390 - 1.790
R-factor	0.1766
Rwork	0.175
R-free	0.20340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.063
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.390	50.000	1.830
High resolution limit [Å]	1.790	4.880	1.800
Rmerge	0.050	0.020	0.492
Rmeas	0.058	0.023	0.579
Rpim	0.030	0.012	0.302
Number of reflections	29761	1537	1462
<I/σ(I)>	10.8
Completeness [%]	100.0	99.9	100
Redundancy	3.8	3.7	3.6
CC(1/2)		0.999	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1 M 2-oxoglutarate, 0.1 M Tris pH 8.5