6AL0
The NZ-1 Fab complexed with the PDZ tandem fragment of A. aeolicus S2P homolog with the PA12 tag inserted between the residues 263 and 267
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-27 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1000 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 141.663, 141.663, 78.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.090 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.183 | 0.808 |
Number of reflections | 25220 | 3024 |
<I/σ(I)> | 12.5 | |
Completeness [%] | 100.0 | |
Redundancy | 17.9 | |
CC(1/2) | 0.993 | 0.924 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15-20% (wt./vol.) PEG 3350, 200mM ammonium citrate (pH 7.0) |