6AKZ
Crystal structure of GlcNAc Inducible Gene 2, GIG2 (DUF1479) from Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.652, 54.545, 74.850 |
| Unit cell angles | 90.00, 105.61, 90.00 |
Refinement procedure
| Resolution | 72.090 - 1.690 |
| R-factor | 0.18223 |
| Rwork | 0.181 |
| R-free | 0.21623 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2csg |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.891 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.090 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.100 | 1.001 |
| Number of reflections | 52149 | 4894 |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 25% PEG 6000, 0.1M Tris pH 8.0, 0.1M MgCl2 |
