6AKV
Crystal structure of LysB4, the endolysin from Bacillus cereus-targeting bacteriophage B4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-29 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | I 41 |
Unit cell lengths | 79.457, 79.457, 77.925 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.092 - 2.400 |
R-factor | 0.1805 |
Rwork | 0.176 |
R-free | 0.22430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vo9 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.785 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.057 | 0.351 |
Rmeas | 0.063 | 0.419 |
Rpim | 0.025 | 0.222 |
Number of reflections | 8863 | 449 |
<I/σ(I)> | 19.66 | 2.36 |
Completeness [%] | 91.5 | 92.4 |
Redundancy | 4.9 | 2.9 |
CC(1/2) | 0.353 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 287.15 | 2.0 M ammonium sulfate, 0.1 M Bis-Tris pH 6.5, 2% polyethylene glycol monomethyl ether 550 (PEGMME 550), 8 mM Tris(2-carboxyethyl)phosphine (TCEP) |