6AKJ

The crystal structure of EMC complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	86.689, 86.689, 105.391
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.538 - 2.700
R-factor	0.2086
Rwork	0.207
R-free	0.24090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wz7
RMSD bond length	0.001
RMSD bond angle	0.384
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	75.075	37.538	2.850
High resolution limit [Å]	2.700	8.540	2.700
Rmerge		0.037	1.336
Rmeas	0.109	0.038	1.385
Rpim	0.027	0.010	0.358
Total number of observations	206285
Number of reflections	13026	467	1856
<I/σ(I)>	18.6	15.6	0.6
Completeness [%]	100.0	98.8	100
Redundancy	15.8	13.9	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	1.6M (NH4)2SO4, 0.1M MES pH 6.5