6AKE
Crystal structure of mouse claudin-3 in complex with C-terminal fragment of Clostridium perfringens enterotoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.600, 66.630, 111.540 |
| Unit cell angles | 90.00, 97.55, 90.00 |
Refinement procedure
| Resolution | 45.900 - 3.600 |
| R-factor | 0.2651 |
| Rwork | 0.264 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3x29 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.564 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.900 | 3.730 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.048 | 1.190 |
| Rmeas | 0.054 | 1.330 |
| Rpim | 0.024 | 0.580 |
| Number of reflections | 15888 | 952 |
| <I/σ(I)> | 14.86 | 1.51 |
| Completeness [%] | 93.9 | 60.68 |
| Redundancy | 5 | 5.3 |
| CC(1/2) | 0.999 | 0.561 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293.15 | Tris-HCl, sodium chloride, magnesium nitrate, PEG 2000 MME, LMNG |
