6AK7
Crystal structure of PPM1K-N94K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2017-10-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 122.784, 122.784, 61.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.445 - 2.605 |
| R-factor | 0.1939 |
| Rwork | 0.192 |
| R-free | 0.22430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4da1 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.500 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 16613 | |
| <I/σ(I)> | 0.3 | |
| Completeness [%] | 99.6 | |
| Redundancy | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris, pH 8.5, 12% PEG 3350, 0.2 M MgCl2, 10 mM Mercaptoethanol |
