6AJS
H109S mutant form of Uracil DNA glycosylase X.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 190 |
| Detector technology | CCD |
| Collection date | 2016-05-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.229, 51.393, 54.444 |
| Unit cell angles | 90.00, 105.21, 90.00 |
Refinement procedure
| Resolution | 28.022 - 1.632 |
| R-factor | 0.163 |
| Rwork | 0.160 |
| R-free | 0.19710 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.937 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.063 | 0.129 |
| Number of reflections | 23431 | 1101 |
| <I/σ(I)> | 56 | |
| Completeness [%] | 97.4 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 2.0M ammonium citrate tribasic pH7.0, 0.1M Bis-Tris propane pH7.0 |
