6AJL
DOCK7 mutant I1836Y complexed with Cdc42
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-12 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 194.550, 105.290, 117.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.638 - 3.230 |
R-factor | 0.1883 |
Rwork | 0.184 |
R-free | 0.25870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6aj4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.242 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.430 |
High resolution limit [Å] | 3.230 | 3.230 |
Rmeas | 0.180 | 0.940 |
Number of reflections | 74614 | 11786 |
<I/σ(I)> | 7.5 | 1.6 |
Completeness [%] | 99.4 | 97.7 |
Redundancy | 3.6 | |
CC(1/2) | 0.991 | 0.635 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG3350, tri-potassium citrate monohydrate |