6AJK

Crystal structure of TFB1M and h45 in homo sapiens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-01-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9790
Spacegroup name	P 43 2 2
Unit cell lengths	122.564, 122.564, 73.067
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.271 - 3.001
R-factor	0.1988
Rwork	0.197
R-free	0.24110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gc5
RMSD bond length	0.007
RMSD bond angle	0.952
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.252	0.099	0.819
Rmeas	0.273	0.108	0.886
Rpim	0.101	0.041	0.322
Number of reflections	11458	1234	1136
<I/σ(I)>	2
Completeness [%]	98.3	96.3	99.6
Redundancy	6.2	5.6	6.5
CC(1/2)		0.994	0.781

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.1M sodium citrate, pH 5.0, 20% PEG 8000