6AIP
Cab2 mutant-H337A complex with phosphopantothenoylcystine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-26 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.097, 112.468, 54.573 |
Unit cell angles | 90.00, 91.12, 90.00 |
Refinement procedure
Resolution | 56.230 - 1.990 |
R-factor | 0.1826 |
Rwork | 0.181 |
R-free | 0.21760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p9o |
RMSD bond length | 0.009 |
RMSD bond angle | 1.415 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.230 | 2.030 |
High resolution limit [Å] | 1.990 | 2.000 |
Rmerge | 0.110 | 0.604 |
Rpim | 0.047 | 0.270 |
Number of reflections | 40420 | 2030 |
<I/σ(I)> | 16.7 | |
Completeness [%] | 96.2 | |
Redundancy | 6.5 | |
CC(1/2) | 0.873 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287 | 200mM ammonium tartrate, 20% PEG 3350 2mM MnCl2 |