6AIE
Crystal structure of a new form of RsmD-like RNA methyl transferase from Mycobacterium tuberculosis determined at 1.74 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-19 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9677 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 95.782, 55.300, 100.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.390 - 1.740 |
R-factor | 0.21211 |
Rwork | 0.211 |
R-free | 0.24260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3p9n |
RMSD bond length | 0.018 |
RMSD bond angle | 1.890 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.800 | 1.770 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.019 | |
Number of reflections | 57366 | 3777 |
<I/σ(I)> | 5.6 | 5.6 |
Completeness [%] | 98.7 | |
Redundancy | 3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2M Potassium acetate, 100mM Tris (PH 7.4) |