6AI1
Structure of the 328-692 fragment of FlhA (D456V)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-08 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.00 |
Spacegroup name | I 41 |
Unit cell lengths | 216.790, 216.790, 64.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.198 - 3.500 |
R-factor | 0.1944 |
Rwork | 0.189 |
R-free | 0.24120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3a5i |
RMSD bond length | 0.011 |
RMSD bond angle | 1.239 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.600 | 3.830 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.137 | 0.449 |
Number of reflections | 19380 | 4609 |
<I/σ(I)> | 7.6 | 3.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG-400, 0.1M HEPES pH 7.5, 0.25M NaCl |