6AI0

Structure of the 328-692 fragment of FlhA (orthorhombic form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-05
Detector	RAYONIX MX-225
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	52.920, 93.080, 187.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.281 - 2.400
R-factor	0.2424
Rwork	0.241
R-free	0.27690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a5i
RMSD bond length	0.003
RMSD bond angle	0.588
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.119	0.421
Number of reflections	36660	3767
<I/σ(I)>	10	3.4
Completeness [%]	99.0	99
Redundancy	7.2	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M HEPES NaOH pH 7.5, 10% (w/v) PEG-8000, 0.4M Ca(OAc)2