6AHP
Structure of the 58-167 fragment of FliL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 |
| Unit cell lengths | 104.201, 104.201, 40.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.101 - 2.100 |
| R-factor | 0.2107 |
| Rwork | 0.205 |
| R-free | 0.25850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A model built by the Robetta server |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.534 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.101 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.056 | 0.435 |
| Number of reflections | 14756 | 11233 |
| <I/σ(I)> | 23.4 | 5.3 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 9.4 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Tris-HCl pH 8.0, 22% (w/v) PEG-4000, 0.2M lithium sulfate |
