6AHO
Crystal structure of Kap114p
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2016-11-27 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.99984 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 116.623, 116.623, 189.015 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.974 - 2.502 |
R-factor | 0.2131 |
Rwork | 0.211 |
R-free | 0.26310 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.258 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.077 | 0.523 |
Rpim | 0.039 | 0.280 |
Number of reflections | 51352 | 4664 |
<I/σ(I)> | 15.1 | 2.2 |
Completeness [%] | 95.6 | 90.5 |
Redundancy | 4.7 | 4 |
CC(1/2) | 0.998 | 0.925 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.43 M citric acid, 0.057 M BIS TRIS propane (pH 5.8), 17% PEG3350, 0.01 M Ethylenediaminetetraacetic acid disodium salt dihydrate, and 3% v/v ethanol |