6AHG
Trimeric structure of concanavalin A from Canavalia ensiformis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 136.461, 136.461, 193.146 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.290 - 2.830 |
| R-factor | 0.24502 |
| Rwork | 0.243 |
| R-free | 0.27919 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ygm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.636 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.290 | 2.980 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Rmerge | 0.098 | 0.457 |
| Number of reflections | 22230 | 3172 |
| <I/σ(I)> | 15.5 | 4.8 |
| Completeness [%] | 99.8 | |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Polyethylene glycol 400, 0.1 M Cadmium chloride hydrate, 0.1 M Sodium acetate trihydrate pH 4.6 |
