6AH8
Marine bacterial prolidase with promiscuous organophosphorus hydrolase activity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2018-05-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97892 |
Spacegroup name | H 3 2 |
Unit cell lengths | 178.854, 178.854, 371.586 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.530 - 2.610 |
Rwork | 0.226 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zwo |
RMSD bond length | 0.005 |
RMSD bond angle | 0.995 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.176 | 0.851 |
Number of reflections | 70044 | 6932 |
<I/σ(I)> | 14.1 | 2 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 9.9 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2 M Ammonium sulfate, 5% PEG400, 100 mM MES pH6.5 |