6AH6
M500V mutant of Coronin coiled coil domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-08 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 117.700, 49.830, 45.330 |
Unit cell angles | 90.00, 112.51, 90.00 |
Refinement procedure
Resolution | 18.120 - 2.500 |
Rwork | 0.270 |
R-free | 0.29870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cx2 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.341 |
Data reduction software | iMOSFLM (7.2.2) |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER (1.8.4_1496) |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.120 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.025 | 0.062 |
Rmeas | 0.035 | 0.088 |
Number of reflections | 8088 | 834 |
<I/σ(I)> | 18.29 | 9.16 |
Completeness [%] | 94.7 | 99.88 |
Redundancy | 2 | 2 |
CC(1/2) | 0.998 | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 297 | Lithium sulfate, ammonium sulphate, sodium citrate 5.6 |