6AGZ

Crystal structure of Old Yellow Enzyme from Pichia sp. AKU4542

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-14
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	46.450, 108.710, 89.930
Unit cell angles	90.00, 91.51, 90.00

Refinement procedure

Resolution	19.690 - 2.000
R-factor	0.2351
Rwork	0.234
R-free	0.25670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.576
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (dev_2210)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	58789
<I/σ(I)>	18
Completeness [%]	97.2
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25%(v/v) PEG 3350, 100mM Tris-HCl (pH 8.0) 200mM ammonium sulfate