6AGW

Pro-domain of Caspase-8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	110
Detector technology	CCD
Collection date	2015-08-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	90.717, 62.807, 52.997
Unit cell angles	90.00, 118.90, 90.00

Refinement procedure

Resolution	29.632 - 2.093
R-factor	0.2255
Rwork	0.221
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.047
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.090	2.100
Number of reflections	15189
<I/σ(I)>	33.3
Completeness [%]	98.5
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	(NH4)2SO4, Cacodylate, NaCl