6AGV
Crystal structure of apo mouse MsrA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-15 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.825, 52.634, 70.670 |
Unit cell angles | 90.00, 118.67, 90.00 |
Refinement procedure
Resolution | 30.680 - 1.620 |
R-factor | 0.1625 |
Rwork | 0.161 |
R-free | 0.18600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.840 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (1.13_2998) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.650 |
High resolution limit [Å] | 1.620 | 1.620 |
Number of reflections | 165785 | |
<I/σ(I)> | 51.72 | 7.03 |
Completeness [%] | 96.9 | 91 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Tris pH 9.0, 0.2M NaCl, 26% polyethylene glycol 3350, 0.1M Sodium Iodide and were used in ratio of 1:1.35 (mouse MsrA and Trx peptide) |