6AGJ
Crystal Structure of EFHA2 in Apo State
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-25 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 72.363, 72.363, 339.751 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.630 - 2.999 |
R-factor | 0.2244 |
Rwork | 0.218 |
R-free | 0.28620 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.519 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | AutoSol |
Refinement software | PHENIX ((dev_2999: 000)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 2.990 | 2.990 |
Number of reflections | 19171 | 885 |
<I/σ(I)> | 22.3 | |
Completeness [%] | 99.7 | |
Redundancy | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M imidazole pH 9.5, 15% ethanol, 0.2 M MgCl2. |