6AGJ

Crystal Structure of EFHA2 in Apo State

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9786
Spacegroup name	P 41 21 2
Unit cell lengths	72.363, 72.363, 339.751
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.630 - 2.999
R-factor	0.2244
Rwork	0.218
R-free	0.28620
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.519
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	AutoSol
Refinement software	PHENIX ((dev_2999: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	2.990	2.990
Number of reflections	19171	885
<I/σ(I)>	22.3
Completeness [%]	99.7
Redundancy	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M imidazole pH 9.5, 15% ethanol, 0.2 M MgCl2.