6AGH

Crystal structure of EFHA1 in Apo-State

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9791
Spacegroup name	P 1
Unit cell lengths	44.712, 63.199, 66.115
Unit cell angles	92.36, 101.75, 96.74

Refinement procedure

Resolution	38.040 - 2.742
R-factor	0.219
Rwork	0.213
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6agj
RMSD bond length	0.005
RMSD bond angle	0.857
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2666: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.740	2.740
Number of reflections	17757	885
<I/σ(I)>	14.48
Completeness [%]	98.4
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20 mM Tris-HCl pH 8.5, 200 mM NaCl, 1 mM EGTA, 2 mM DTT