6AG8
Crystal structure of Maltose O-acetyltransferase from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 3 |
| Unit cell lengths | 62.104, 62.104, 81.128 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.910 - 1.340 |
| R-factor | 0.17239 |
| Rwork | 0.171 |
| R-free | 0.19020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ic7 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.778 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.360 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rpim | 0.029 | 0.187 |
| Number of reflections | 78711 | 3791 |
| <I/σ(I)> | 34.66 | |
| Completeness [%] | 99.5 | |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% Poly Ethylene glycol 3350, 0.1M Tris (pH 8.0), 0.2M NaCl |
