6AG4
Crystal structure of Ard1 N-terminal acetyltransferase H88A/E127A mutant from Sulfolobus solfataricus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2014-03-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.335, 53.148, 74.505 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.967 - 2.256 |
R-factor | 0.1692 |
Rwork | 0.163 |
R-free | 0.22850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r3k |
RMSD bond length | 0.007 |
RMSD bond angle | 1.022 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.360 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 8167 | |
<I/σ(I)> | 24.6 | |
Completeness [%] | 98.1 | |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 283 | 0.75M ammonium sulfate, 0.1M sodium acetate trihydrate, pH 4.2, 0.1M lithium sulfate |