6AG2
uPA-HMA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL18U |
Synchrotron site | NFPSS |
Beamline | BL18U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | H 3 |
Unit cell lengths | 120.407, 120.407, 42.017 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.759 - 1.770 |
R-factor | 0.22123 |
Rwork | 0.220 |
R-free | 0.25221 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.295 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.780 | 1.780 |
Number of reflections | 21364 | |
<I/σ(I)> | 17.2 | |
Completeness [%] | 97.3 | |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 2.0M ammonium sulfate, 50mM sodium citrate (pH 4.6), 5% PEG 400 |