6AF4
Toxin-Antitoxin module from Streptococcus pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2016-09-28 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.903, 73.403, 98.321 |
Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
Resolution | 30.040 - 2.650 |
R-factor | 0.218 |
Rwork | 0.216 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6af3 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.135 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 45671 | |
<I/σ(I)> | 23.7 | |
Completeness [%] | 97.0 | |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20%(w/v) PEG 1000, 0.1M potassium phosphate monobasic/sodium phosphate dibasic, pH 6.2, 0.2M sodium chloride |