6AF4

Toxin-Antitoxin module from Streptococcus pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	293
Detector technology	CCD
Collection date	2016-09-28
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	74.903, 73.403, 98.321
Unit cell angles	90.00, 90.08, 90.00

Refinement procedure

Resolution	30.040 - 2.650
R-factor	0.218
Rwork	0.216
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6af3
RMSD bond length	0.006
RMSD bond angle	1.135
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	45671
<I/σ(I)>	23.7
Completeness [%]	97.0
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20%(w/v) PEG 1000, 0.1M potassium phosphate monobasic/sodium phosphate dibasic, pH 6.2, 0.2M sodium chloride