6AF3
Toxin-Antitoxin module from Streptococcus pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2016-05-31 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.575, 67.038, 87.717 |
Unit cell angles | 90.00, 94.22, 90.00 |
Refinement procedure
Resolution | 49.795 - 2.803 |
R-factor | 0.2263 |
Rwork | 0.220 |
R-free | 0.33330 |
Structure solution method | SAD |
RMSD bond length | 0.028 |
RMSD bond angle | 3.126 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rpim | 0.043 | 0.480 |
Number of reflections | 41486 | 2066 |
<I/σ(I)> | 24.8 | |
Completeness [%] | 99.9 | |
Redundancy | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20%(w/v) PEG 1000, 0.1M potassium phosphate monobasic/sodium phosphate dibasic, pH 6.2, 0.2M sodium chloride |