6AF2
Crystal structure of N-terminus deletion mutant of Mycobacterium avium diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.025, 104.837, 111.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.845 - 3.001 |
| R-factor | 0.2107 |
| Rwork | 0.208 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ano |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.549 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.113 | 0.253 |
| Number of reflections | 16834 | 485 |
| <I/σ(I)> | 12.1 | 2.2 |
| Completeness [%] | 89.4 | 54 |
| Redundancy | 9.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 50 mM Hepes (pH 7.6), 0.2 M CaCl2, 28% PEG 400, 2mM spermine |
