6AF2
Crystal structure of N-terminus deletion mutant of Mycobacterium avium diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.025, 104.837, 111.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.845 - 3.001 |
R-factor | 0.2107 |
Rwork | 0.208 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ano |
RMSD bond length | 0.011 |
RMSD bond angle | 1.549 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.113 | 0.253 |
Number of reflections | 16834 | 485 |
<I/σ(I)> | 12.1 | 2.2 |
Completeness [%] | 89.4 | 54 |
Redundancy | 9.5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 50 mM Hepes (pH 7.6), 0.2 M CaCl2, 28% PEG 400, 2mM spermine |