6AEP
Crystal structure of the ssDNA-binding domain of DnaT from Salmonella enterica Serovar Typhimurium LT2 at 1.84 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2018-04-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.975 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 57.282, 93.526, 68.902 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.737 - 1.844 |
R-factor | 0.2172 |
Rwork | 0.215 |
R-free | 0.24850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ou6 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.789 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.910 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.043 | 0.284 |
Number of reflections | 15843 | 1479 |
<I/σ(I)> | 28.12 | 4.08 |
Completeness [%] | 97.1 | 91.2 |
Redundancy | 4.8 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.6 M Magnesium Sulfate, 100 mM MES Sodium Salt pH 6.5 |