6AEP
Crystal structure of the ssDNA-binding domain of DnaT from Salmonella enterica Serovar Typhimurium LT2 at 1.84 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2018-04-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.975 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 57.282, 93.526, 68.902 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.737 - 1.844 |
| R-factor | 0.2172 |
| Rwork | 0.215 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ou6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.789 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.910 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.043 | 0.284 |
| Number of reflections | 15843 | 1479 |
| <I/σ(I)> | 28.12 | 4.08 |
| Completeness [%] | 97.1 | 91.2 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.6 M Magnesium Sulfate, 100 mM MES Sodium Salt pH 6.5 |
