6AE9
X-ray structure of the photosystem II phosphatase PBCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2015-05-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 166.407, 166.407, 46.983 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.700 - 1.470 |
| R-factor | 0.1606 |
| Rwork | 0.160 |
| R-free | 0.17890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j82 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.825 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.520 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.057 | 0.397 |
| Number of reflections | 81886 | 7948 |
| <I/σ(I)> | 26.3 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.3 M MgCl2, 0.1 M Tris-HCl, pH 8.1, 29% PEG4000, 5% v/v Jeffamine M-600 |
