6AE2

Crystal structure of Csm3 of the type III-A CRISPR-Cas effector complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2012-11-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9788
Spacegroup name	P 41 21 2
Unit cell lengths	78.331, 78.331, 182.686
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.455 - 2.701
R-factor	0.2128
Rwork	0.211
R-free	0.24280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.592
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP (10.2.31)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.080	0.041	0.466
Total number of observations	227320
Number of reflections	16335	910	767
<I/σ(I)>	18.8
Completeness [%]	99.7	95.8	100
Redundancy	13.9	11.4	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	180 mM di-ammonium tartrate, 18% (w/v) PEG 3350, 3% (w/v) PEG 1500