6AD8

Crystal structure of the E148D mutant CLC-ec1 in 50 mM bromide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-05-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9198
Spacegroup name	C 1 2 1
Unit cell lengths	231.440, 100.080, 172.550
Unit cell angles	90.00, 132.61, 90.00

Refinement procedure

Resolution	50.040 - 3.300
R-factor	0.23
Rwork	0.227
R-free	0.28370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ene
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.500	3.420
High resolution limit [Å]	3.300	3.300
Rmerge	0.080	0.570
Number of reflections	83063	6039
<I/σ(I)>	5.6	1.2
Completeness [%]	96.6
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	PEG400 20%(w/v), 100mM tris-SO4, 20mM Na/K tartrate, 50mM NaBr