6AD8
Crystal structure of the E148D mutant CLC-ec1 in 50 mM bromide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-05-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9198 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 231.440, 100.080, 172.550 |
Unit cell angles | 90.00, 132.61, 90.00 |
Refinement procedure
Resolution | 50.040 - 3.300 |
R-factor | 0.23 |
Rwork | 0.227 |
R-free | 0.28370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ene |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.500 | 3.420 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.080 | 0.570 |
Number of reflections | 83063 | 6039 |
<I/σ(I)> | 5.6 | 1.2 |
Completeness [%] | 96.6 | |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | PEG400 20%(w/v), 100mM tris-SO4, 20mM Na/K tartrate, 50mM NaBr |