6AD7
Crystal structure of the E148D mutant CLC-ec1 in 20 mM bromide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-05-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9198 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 233.181, 97.610, 170.800 |
| Unit cell angles | 90.00, 131.79, 90.00 |
Refinement procedure
| Resolution | 49.036 - 2.950 |
| R-factor | 0.2251 |
| Rwork | 0.223 |
| R-free | 0.26940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ene |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.036 | 3.055 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.060 | 0.550 |
| Number of reflections | 113927 | 11451 |
| <I/σ(I)> | 6.2 | 1.3 |
| Completeness [%] | 96.2 | 96.7 |
| Redundancy | 6.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | PEG400 19%(W/v), 100mM Tris-SO4, 20mM Na/K tartrate, 20 mM NaBr |
