6ACX
Crystal structure of Mycobacterium smegmatis Mfd in complex with ADP + Pi at 3.5 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-03-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.659, 160.440, 214.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.103 - 3.500 |
| R-factor | 0.2286 |
| Rwork | 0.226 |
| R-free | 0.27310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ac8 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.524 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575: 000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.730 | 3.660 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.200 | 0.594 |
| Rmeas | 0.245 | 0.711 |
| Rpim | 0.104 | 0.305 |
| Number of reflections | 35989 | 4329 |
| <I/σ(I)> | 6.3 | 2.4 |
| Completeness [%] | 96.8 | 97.1 |
| Redundancy | 5.1 | 4.9 |
| CC(1/2) | 0.982 | 0.353 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 295 | 100mM HEPES sodium pH 7.2, 0.2M Na2So4, 20% PEG3350 |
