6ACP
histone lysine desuccinylase Sirt5 in complex with succinyl peptide H4K91
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.078, 41.267, 61.334 |
| Unit cell angles | 90.00, 108.37, 90.00 |
Refinement procedure
| Resolution | 58.210 - 2.300 |
| R-factor | 0.20721 |
| Rwork | 0.203 |
| R-free | 0.28510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ace |
| RMSD bond length | 0.092 |
| RMSD bond angle | 1.664 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.210 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.149 | 0.412 |
| Number of reflections | 12138 | 2291 |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 99.0 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 0.1 M BIS-TRIS (pH 5.5), 25% w/v PEG 3350 |
