6ACP
histone lysine desuccinylase Sirt5 in complex with succinyl peptide H4K91
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.078, 41.267, 61.334 |
Unit cell angles | 90.00, 108.37, 90.00 |
Refinement procedure
Resolution | 58.210 - 2.300 |
R-factor | 0.20721 |
Rwork | 0.203 |
R-free | 0.28510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ace |
RMSD bond length | 0.092 |
RMSD bond angle | 1.664 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.210 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.149 | 0.412 |
Number of reflections | 12138 | 2291 |
<I/σ(I)> | 8.5 | |
Completeness [%] | 99.0 | |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 0.1 M BIS-TRIS (pH 5.5), 25% w/v PEG 3350 |