6ACF
structure of leucine dehydrogenase from Geobacillus stearothermophilus by cryo-EM
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | JEOL KYOTO-3000SFF |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 1.6 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3 |
| Software | EMAN2,RELION,PHENIX,CTFFIND |
| Symmetry | D4 |
| CTF correction | PHASE FLIPPING ONLY |
